The US government has added a dozen more Chinese firms to its list of restricted trade partners, citing national security and foreign policy concerns.
According to Washington, some of the companies are assisting the Chinese military's quantum computing development.
This latest action comes as tensions between the United States and China rise over Taiwan's status and other concerns.
Trade was one of the topics covered during a virtual summit between the presidents of both countries earlier this month.
A total of 27 new entities from China, Japan, Pakistan, and Singapore were added to the list.
Separately, the Moscow Institute of Physics and Technology was added to the department's list of military end-users, however, the entry provided no more information other than the fact that it had developed military equipment.
The new listings would help prevent American technology from being used to promote Chinese and Russian "military advances and non-proliferation concerns, such as Pakistan's unguarded nuclear operations or ballistic missile program," Commerce Secretary Gina Raimondo said in a statement.
Potential suppliers to enterprises on the list must now apply for a license before they may sell to them, and applications are likely to be refused.
Huawei, the Chinese telecoms giant, was added to the list in 2019 following allegations that it constituted a threat to US national security.
It was cut off from several of its main suppliers as a result of the relocation, making it harder for the business to make mobile phones.
Previously, the Chinese government denied involvement in industrial espionage.